Susceptibility studies indicated that overproduction of the P. aeruginosa strain, PAO4089, that is deficient in producing chromosomal β-lactamase. These data are consistent with the conclusion that PBP3 has to achieve a certain level of saturation, with β-lactams targeted to this protein, to result in cell inhibition or death.ĪB - By using a broad-host-range vector, pUCP27, the Pseudomonas aeruginosa and Escherichia coli pbpB genes, which encode penicillin-binding protein 3 (PBP3), were separately overexpressed in a P. coli PBP3, with the exception that there was no influence on the MICs or minimal bactericidal concentrations of cefsulodin and cefepime, which have very low affinities for E. Similar results were obtained with PAO4089 overproducing E. aeruginosa PBP3 in PAO4089 did not affect susceptibility to PBP1-targeted cephaloridine or PBP2-targeted imipenem. aeruginosa PBP3 in PAO4089 resulted in twofold-increased resistance to aztreonam, fourfold-increased resistance to cefepime and cefsulodin, and eightfold-increased resistance to ceftazidime, whereas overproduction of the P. N2 - By using a broad-host-range vector, pUCP27, the Pseudomonas aeruginosa and Escherichia coli pbpB genes, which encode penicillin-binding protein 3 (PBP3), were separately overexpressed in a P. T1 - Susceptibility to β-lactam antibiotics of Pseudomonas aeruginosa overproducing penicillin-binding protein 3Ĭopyright 2020 Elsevier B.V., All rights reserved. These data are consistent with the conclusion that PBP3 has to achieve a certain level of saturation, with β-lactams targeted to this protein, to result in cell inhibition or death.",
These data are consistent with the conclusion that PBP3 has to achieve a certain level of saturation, with β-lactams targeted to this protein, to result in cell inhibition or death.Ībstract = "By using a broad-host-range vector, pUCP27, the Pseudomonas aeruginosa and Escherichia coli pbpB genes, which encode penicillin-binding protein 3 (PBP3), were separately overexpressed in a P. Read more about user accounts at EcoliWiki membership.By using a broad-host-range vector, pUCP27, the Pseudomonas aeruginosa and Escherichia coli pbpB genes, which encode penicillin-binding protein 3 (PBP3), were separately overexpressed in a P. coli targets click here.ĮcoliWiki members can use EcoliWiki in ways that are not available to unregistered users. Tell us what you want to see on EcoliWikiĮcoliWiki participates in the RefGenome project.Add new pages about topics you're interested in.There are lots of ways you can help us make EcoliWiki better: Identity and Function of a Large Gene Network Underlying Mutagenic Repair of DNA BreaksĮcoliWiki is for everyone. Some sample queries are listed below, and you can always read more about searching on our Help pages. You can also browse our categories if you want to see what's here. in the long run.įind information within EcoliWiki by using the Search box to the left. Our goal for EcoliWiki is to generate community-based pages aboutĮverything related to non-pathogenic E. PortEco's (formerly EcoliHub) subsystem for community annotation.